Kylie represented Australia in distance freestyle swimming. She made her Olympic debut at the 2008 Beijing Games, winning gold in the 4 x 200m freestyle relay and taking sixth in the 800m freestyle.

She followed this up with multiple Commonwealth Games and World Championship medals before winning a silver medal at the 2012 London Olympic Games.

After retiring in 2016, Kylie became a coach and mentor and is an ambassador for various charities and organisations.