The ergogenic nature of glycerol has been investigated according to its effect on fluid retention, which has been shown to positively influence thermoregulatory function, cardiovascular responses and, hence, athletic performance.

Research on impact of glycerol on thermoregulation and performance have provided mixed results but some studies, including trials conducted at the AIS, have shown benefits to performance of moderate-high intensity exercise performed in the heat.

A meta-analysis concluded that the use of glycerol-induced hyperhydration in hot conditions provided a small (3% power output, effect size 0.35) but worthwhile enhancement to prolonged exercise performance above hyperhydration with water.